These premium antibodies are lab-validated using strict testing criteria to ensure superior performance in western blotting detection. PrecisionAb Validated Western Blotting Antibodies.Loading buffers for protein or nucleic acid samples, detergents, ampholytes, and gel-casting buffers.įrom basic to high-voltage capacity, our power supplies provide simple programming in a compact, stackable case. Protein Electrophoresis Buffers and Reagents.Our electrophoresis chambers enable rapid, high-resolution protein separation on precast or handcast gels over a variety of different gel sizes. Choose SDS-PAGE and native PAGE gels, convert to TGX Precast Gels, or choose specialized gel chemistries. Prestained and unstained molecular weight standards for protein electrophoresis applications including SDS-PAGE, western blotting, 2-D PAGE, and isoelectric focusing (IEF).įind the right Bio-Rad protein gel for your application. Protein Ladders and Standards (Markers).If you are in the process of customizing Dynabeads His-Tag Isolation and Pulldown beads in a commercial product or service or need a larger bulk volume of this product, please contact us at or see our Dynabeads OEM page. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility making them an ideal choice for commercial supply.
Biorad magnetic beads antibody reactivity iso#
Our manufacturing sites are ISO 13485-certified. This process can be automated using any of our KingFisher sample purification systems or liquid handlers. The 1-μm magnetic beads are ideal for high-throughput enrichment. Dynabeads His-Tag Isolation and Pulldown beads are 1-m monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets. This can later be used for applications requiring a strongly bound target, without the risk of reduced target yield.Īutomation-ready for use with KingFisher purification systems It will not elute in the commonly used elution buffers for IMAC. This oxidizes cobalt to the Co3+ state, making the connection between the bead and the target permanent. The bead carries cobalt Co2+ ions, so it is possible to cement the bond between the bead and the target just by using mildly oxidative conditions, such as 10 mM hydrogen peroxide for 30–60 minutes.
In addition to being a great tool for protein pulldown and isolation, the Dynabeads His-Tag Isolation and Pulldown beads can serve as a platform for target immobilization. After washing, the target is eluted off and ready for downstream applications. The beads are added to the solution containing the target and mixed for 5-10 minutes. Since the bead surface is loaded with cobalt ions, it is also possible to make a stable and lasting bond with the target molecule through oxidation if the downstream applications require it. Using the principles of immobilized metal affinity binding together with the inert surface of Dynabeads magnetic beads helps ensure a clean capture with extremely low carryover of unwanted proteins. They are also suited for other applications with high signal-to-noise requirements. Good signal-to-noise for numerous applicationsĭynabeads His-Tag Isolation and Pulldown beads are an ideal tool for isolation of tagged recombinant proteins. High IgG binding affinity with low nonspecific binding and reduced antibody consumption. Automation-ready-automation protocols available with the KingFisher Purification instruments Unique bead surface chemistry ensures optimal antibody orientation for increased antigen binding efficiency.Flexibility-flexible protocols with gentle and efficient liquid-phase reaction kinetics.Efficiency-fast binding kinetics and high binding capacity.Performance-high sensitivity and reproducibility securing consistent results.Dynabeads His-Tag Isolation and Pulldown beads can also be a great starting point for other advanced experimental approaches where solid magnetic support is needed.īenefits of Dynabeads His-Tag and Pulldown beads include:
Biorad magnetic beads antibody reactivity manual#
They enable single-step isolation of tagged molecules in manual or automated workflows that helps ensure good recovery of the target. Their small but uniform bead size presents a high surface area per mg beads and a correspondingly high capacity for the target molecule. Dynabeads™ His-Tag Isolation and Pulldown beads are 1-μm monosized, monodispersed, superparamagnetic beads with an NTA-Cobalt-based ligand which has a high affinity towards His-tags and similar structures on molecular targets.
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